| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Warrenton High School | A | Besser | Howard | Grooms | Marschel | 10:55.38 | Verified |
| 2 | St. Charles West High School | A | Jackson | Everley | Jackson | Malinee | 11:19.28 | Verified |
| 3 | St. Charles High School | A | Gilkison | Rayburn | Coffman | Schiffer | 12:09.47 | Verified |
| 4 | Orchard Farm High School | A | Bauer | Hulbert | Schwierjohn | Cattoor | 12:11.62 | Verified |
| 5 | Winfield High School | A | Schleeper | Mackey | Gerholdt | Edwards | 12:39.15 | Verified |
