| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Columbia Smithton MS | A | Backues | Brown | Swofford | Sweeney | 52.74 | Verified |
| 3 | Columbia West MS | A | Taylor | Riley | Laird | Frederick | 50.72 | Verified |
| 4 | Columbia West MS | B | Almquist | Austin | Knarr | Williams | 50.72 | Verified |
