| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ft. Zumwalt West High School | A | Lo | Parker | Boatman | Copelin | 8:10.17 | Verified |
| 2 | Rock Bridge High School | A | Kemey | Jokerst | Summerall | Hauser | 8:17.52 | Verified |
| 3 | Hickman High School | A | Abrams | Littrell | McGarvey | Yoseph | 8:19.35 | Verified |
| 4 | John Burroughs High School | A | Mallady | Martin | Spetnagel | Doty | 8:38.14 | Verified |
| 5 | Francis Howell High School | A | Murphy | Mcguire | Filipek | Nordgaarden | 8:57.00 | Unverified: |
| 6 | Hazelwood Central High School | A | Ellis | Feaman | Quinn | Stiles | 9:55.39 | Verified |
