Section 1 of 3
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CLEMONS, Brooke | SR | Francis Howell High School | 1:10.68 | |
| 2 | SCHIERMEIER, Khyla | SO | Francis Howell High School | 30.65 | Verified |
| 3 | FRENCH, Mikel | SO | Ritenour High School | 30.09 | Verified |
| 4 | RIDER, Lyla | SO | Ft. Zumwalt West High School | 29.72 | Verified |
| 5 | SIMMONS, Manna | SR | Francis Howell High School | 29.97 | Verified |
| 6 | WHITE, Kaylee | SR | Ft. Zumwalt West High School | 30.60 | Verified |
| 7 | CALICUTT, Chantel | SO | Ritenour High School | 32.66 | Verified |
Section 2 of 3
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ESTES, Azariah | JR | Ritenour High School | 29.26 | Verified |
| 2 | CONCANNON, Sophia | SO | Rock Bridge High School | 28.52 | Verified |
| 3 | MARTYN, Phoebe | JR | John Burroughs High School | 28.25 | Unverified: |
| 4 | MAYS, Jillian | SR | John Burroughs High School | 27.91 | Verified |
| 5 | HYDE, Kenyae | JR | Hickman High School | 28.05 | Unverified: |
| 6 | O'BRIEN, Mary | JR | John Burroughs High School | 28.48 | Verified |
| 7 | JONES, Lauren | JR | Francis Howell North High School | 29.15 | Verified |
| 8 | NGUYEN, Peyton | SO | Francis Howell High School | 29.58 | Verified |
Section 3 of 3
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MCKENZIE, Mya | SO | Hazelwood Central High School | 27.86 | Verified |
| 2 | SMITH, Caitlyn | JR | Hickman High School | 27.59 | Verified |
| 3 | PRIDEMORE, Brooke | SR | Ft. Zumwalt West High School | 27.09 | Verified |
| 4 | PORTER, Zakiya | JR | Rock Bridge High School | 25.15 | Verified |
| 5 | DONABY, Iyonna | SR | Hickman High School | 26.48 | Verified |
| 6 | CRANE, Sunday | SO | Rock Bridge High School | 27.31 | Verified |
| 7 | MAPP, Nina | JR | Hazelwood Central High School | 27.68 | Verified |
| 8 | BELL, Oryel | JR | Hazelwood Central High School | 27.88 | Verified |
