| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Francis Howell High School | A | Schiermeier | Simmons | Jambois | Nguyen | 53.10 | Verified |
| 3 | Hazelwood Central High School | A | Futrell | Mckenzie | Mapp | Ray | 51.86 | Unverified: Holt Invitational |
| 4 | Rock Bridge High School | A | Rush | Crane | Barreto-Gentry | Nunnelly | 50.09 | Verified |
| 5 | Hickman High School | A | Donaby | Hyde | Nennie | Orr | 50.61 | Verified |
| 6 | John Burroughs High School | A | Martyn | Mays | Harris | Elliott | 52.80 | Verified |
| 7 | Ft. Zumwalt West High School | A | Moresi | Loggins | Mueller | White | 54.95 | Verified |
