| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Rock Bridge High School | A | Parsons | Walker | Steuber | Barrett | 9:57.74 | Verified |
| 2 | John Burroughs High School | A | Spetnagel | Wagner | Brown | Nichols | 10:13.75 | Verified |
| 3 | Ft. Zumwalt West High School | A | Lehmbeck | Maupin | Madrid | Linville | 10:28.97 | Verified |
| 4 | Francis Howell High School | A | Shinabery | Meyer | Coulter | Halstead | 10:30.88 | Verified |
| 5 | Hickman High School | A | Henderson | Martin | McEuen | Oh | 11:00.61 | Verified |
