| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ft. Zumwalt North High School | A | Landrum | Page | Friend | Bennett | 4:00:00.00 | Unverified: |
| 2 | Timberland High School | A | Kimberlin | Roettger | Rushing | Dahuja | 4:07.00 | Unverified: |
| 3 | Hazelwood Central High School | A | Withers | Davis | Johnson | Harris | 3:57.30 | Verified |
| 4 | Ft. Zumwalt South High School | A | Walker | Moore | Frost | Joiner | 3:45.93 | Verified |
| 5 | Francis Howell High School | A | 3:56.00 | Unverified: | ||||
| 6 | Francis Howell High School | B | 4:00.00 | Unverified: | ||||
| 7 | Francis Howell Central High Scho | A | DeVilder | Jones | Hedgecorth | Layman | 4:55.00 | Unverified: |
