| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ft. Zumwalt South High School | A | Holman | Hart | Myers | Whitten | 4:58.31 | Unverified: |
| 2 | Francis Howell High School | A | Novak | Young | Chappell | Brady | 4:45.00 | Unverified: |
| 3 | Parkway South High School | A | Swaminathan | Vlasich | Rojas | Ullom | 4:36.60 | Unverified: Park tri |
| 4 | Ft. Zumwalt North High School | B | Maier | Maurer | Vandemark | Arnold | NT | Verified |
| 5 | Ft. Zumwalt North High School | A | Norman | Davis | Luecke | Posadas | 4:35.06 | Verified |
| 6 | Eureka High School | A | 4:40.00 | Unverified: | ||||
| 7 | Eureka High School | B | 4:46.10 | Unverified: | ||||
| 8 | Francis Howell Central High Scho | A | Bannon | Watkins | Creighton | Mason | 5:30.00 | Unverified: |
