Section 1 of 2
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Francis Howell High School | B | Nelson | Garbarini | Ball | Smith | 1:02.00 | Unverified: |
| 4 | Francis Howell Central High Scho | A | Young | Schriber | Johnson | Mason | 59.00 | Unverified: |
| 5 | Timberland High School | A | Baynes | Niffen | Kiner | Diaz | NT | Unverified: |
| 6 | Parkway South High School | B | Jumps | Rosewell | Ley | Scott | 1:03.19 | Verified |
Section 2 of 2
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hazelwood Central High School | A | Adekunle | Edwards | Mays | Randle | NT | Unverified: |
| 2 | Ft. Zumwalt South High School | A | Chatman | Motley | O'Neal | Bass | 56.10 | Unverified: |
| 3 | Eureka High School | A | 55.94 | Unverified: | ||||
| 4 | Ft. Zumwalt North High School | A | Schroeder | Michel | Holloway | Soener | 55.20 | Verified |
| 5 | Ft. Zumwalt North High School | B | Porter | Forness | Miazga | Strong | 55.20 | Verified |
| 6 | Parkway South High School | A | Wilson | Ullom | Vlasich | Jones | 56.09 | Unverified: |
| 7 | Francis Howell High School | A | Schiermeier | Bishop | Walton | Persons | 57.10 | Unverified: |
| 8 | Eureka High School | B | 58.20 | Unverified: |
