| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Clair High School | A | Busse | Kern | Murphy | Smith | 3:53.31 | Verified |
| 2 | Sullivan High School | A | Kirk | Goly | Walker | Krygiel | NT | Verified |
| 3 | Owensville High School | A | Breedlove | Meyer | Whelan | Payne | 3:38.38 | Verified |
| 4 | Union High School | A | Orozco | Burke | Smith | Neely | 3:34.99 | Verified |
| 5 | St. James High School | A | Die | Redburn | Redburn | Altis | 3:38.13 | Verified |
| 6 | Hermann High School | A | Waechter | Gleeson | Giles | Terry | 3:39.70 | Verified |
| 7 | Pacific High School | A | Sparks | Haley | Hunkins | Hoffman | 3:42.06 | Verified |
| 8 | New Haven High School | A | Homeyer | Liggett | Romo-Vazquez | Williams | 3:55.48 | Verified |
