| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BRAKEFIELD, Anna | SO | Union High School | 11:51.70 | Verified |
| 2 | UTHLAUT, Amelia | 9 | Hermann High School | 11:54.27 | Verified |
| 3 | GIBSON, Mekayla | SR | Owensville High School | 11:57.03 | Verified |
| 4 | MCINTYRE, Emma | SR | New Haven High School | 12:16.24 | Verified |
| 5 | MENKE, Katy | SO | Hermann High School | 12:39.50 | Verified |
| 6 | PERONA, Vanessa | 9 | St. James High School | 13:29.33 | Verified |
| 7 | HINGST, Heidi | SR | Hermann High School | 13:31.20 | Verified |
| 8 | OTTEN, Caroline | SR | New Haven High School | 13:47.16 | Verified |
| 9 | MEHRINGER, Olivia | 9 | Union High School | 13:56.76 | Verified |
| 10 | VAUGHN, Carly | SO | Pacific High School | 14:31.77 | Verified |
| 11 | LOEB, Hayden | JR | Owensville High School | 14:37.37 | Verified |
| 12 | WEIRICH, Kyah | JR | Owensville High School | 14:54.48 | Verified |
