| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GILES, Peter | SR | Hermann High School | 2:07.27 | Verified |
| 2 | BREEDLOVE, Jacob | JR | Owensville High School | 2:07.40 | Verified |
| 3 | TERRY, Austin | SR | Hermann High School | 2:07.57 | Verified |
| 4 | GILES, Michael | SR | Hermann High School | 2:07.69 | Verified |
| 5 | PICKARD, Bryson | SO | Union High School | 2:07.78 | Verified |
| 6 | HERBST, William | SO | Union High School | 2:08.51 | Verified |
| 7 | HALEY, Collin | JR | Pacific High School | 2:11.20 | Verified |
| 8 | HOEKEL, Gabriel | JR | Union High School | 2:13.10 | Verified |
| 9 | WALKER, Chase | JR | Sullivan High School | 2:14.41 | Verified |
| 10 | WILLIAMS, Logan | JR | New Haven High School | 2:14.67 | Verified |
| 11 | KIRK, Aiden | JR | Sullivan High School | 2:14.75 | Verified |
| 12 | GARVER, Cody | JR | St. James High School | 2:23.68 | Verified |
| 13 | BEAGLE, Caden | SO | St. Clair High School | 2:25.49 | Verified |
| 14 | DEARDEUFF, Zachary | SR | St. James High School | 2:29.57 | Verified |
| 15 | TOBBEN, Austin | SO | St. Clair High School | 2:31.77 | Verified |
| 16 | SCHULTZ, Quinn | 9 | St. James High School | 2:32.76 | Verified |
