| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MILLER, Morgan | JR | Hermann High School | 2:33.11 | Verified |
| 2 | JONES, Maggie | SR | Sullivan High School | 2:35.15 | Verified |
| 3 | BRAKE, Kelsey | 9 | Union High School | 2:36.50 | Verified |
| 4 | GIBSON, Mekayla | SR | Owensville High School | 2:38.29 | Verified |
| 5 | CLARK, Jessi | SR | Union High School | 2:39.18 | Verified |
| 6 | PRADA, Katie | JR | Pacific High School | 2:40.36 | Verified |
| 7 | RETHEMEYER, Hannah | SR | New Haven High School | 2:43.73 | Verified |
| 8 | ANDING, Jenna | SR | Pacific High School | 2:44.16 | Verified |
| 9 | HALMICK, Loren | JR | Sullivan High School | 2:44.49 | Verified |
| 10 | LANGENBERG, Madison | SR | New Haven High School | 2:44.97 | Verified |
| 11 | MILLER, Melodi | SO | St. Clair High School | 2:46.87 | Verified |
| 12 | HINGST, Heidi | SR | Hermann High School | 2:46.94 | Verified |
| 13 | ELLA, Edsel | JR | St. Clair High School | 2:53.44 | Verified |
| 14 | WEIRICH, Kyah | JR | Owensville High School | 2:54.10 | Verified |
| 15 | HALMICK, Adia | JR | Sullivan High School | 3:01.70 | Verified |
