| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MAYBERRY, Breanna | 9 | Sullivan High School | 2:50.14 | Verified |
| 2 | SCHATZL, Maria | 9 | Sullivan High School | 2:55.71 | Verified |
| 3 | CARVER, Tressa | SO | New Haven High School | 2:59.68 | Verified |
| 4 | GOODIN, Karen | 9 | St. James High School | 2:59.93 | Verified |
| 5 | NIEMAN, Haleigh | 9 | New Haven High School | 3:04.95 | Verified |
| 6 | KINKEAD, Emily | JR | St. James High School | 3:08.65 | Verified |
| 7 | CRONIN, Elaina | 9 | New Haven High School | 3:17.92 | Verified |
| 8 | THACKER, Hannah | SR | St. Clair High School | 3:24.73 | Verified |
| 9 | GOOCH, Cassidy | 9 | Owensville High School | 3:27.64 | Verified |
| 10 | LANGEL, Maggie | SO | Sullivan High School | NT | Unverified: |
