| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | MAUNTEL, Kyra | 9 | New Haven High School | 1:12.40 | Verified |
| 3 | CAUSEY, Molly | 9 | Hermann High School | 1:01.88 | Verified |
| 4 | ROOD, Jill | 9 | Hermann High School | 59.98 | Verified |
| 5 | ODEN, Hadley | JR | Hermann High School | 1:01.62 | Verified |
| 6 | CHANDLER, Alyssa | 9 | Pacific High School | 1:02.94 | Verified |
| 7 | MASTIN, Sabren | SO | Sullivan High School | 1:13.50 | Verified |
