| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Union High School | A | Sansom | Pope | Clary | Stowe | 49.96 | Unverified: Sullivan 9/10 |
| 4 | Sullivan High School | A | Cain | Anderson | Cates | Rodriguez | 45.07 | Verified |
| 5 | St. James High School | A | Bullock | Chirico | Hinderhan | Hughes | 45.29 | Verified |
| 6 | Owensville High School | A | Bialczyk | McDaniel | Marquez | Nicholas | 52.83 | Unverified: Sullivan 9-10 Invite |
