| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Sullivan High School | A | Volkert | Hatton | Davis | Nolie | 55.25 | Verified |
| 2 | New Haven High School | A | Baker | Faris | Faris | Kubiak | 54.95 | Verified |
| 3 | Pacific High School | A | Wallace | Blackburn | Parry | Haddox | 53.99 | Verified |
| 4 | Hermann High School | A | Dixon | Whithaus | Worland | Schulte | 53.74 | Verified |
| 5 | Union High School | A | Miner | Rinne | Bush | Eagan | 53.99 | Verified |
| 6 | St. James High School | A | Light | Satterfield | Crocker | Bullock | 54.49 | Verified |
| 7 | Owensville High School | A | Angell | Richardson | Reynolds | Watkins | 55.10 | Unverified: Hermann Invitaitonal |
| 8 | St. Clair High School | A | Cox | Mutschler | Murray | Rampani | NT | Unverified: |
