| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Pacific High School | A | Prokawski | Chandler | Tomlinson | Murphy | 1:03.99 | Unverified: |
| 3 | St. James High School | A | Rogers | Carroll | Patel | Mullins | 58.90 | Unverified: |
| 4 | Hermann High School | A | Rood | McNeill | Crawford | Terry | 57.74 | Unverified: |
| 5 | Sullivan High School | A | Bogert | Bell | Mastin | Carpenter | 58.79 | Unverified: |
| 6 | Owensville High School | A | Ritter | Baguio | Farris | Baker | 1:01.58 | Unverified: Sullivan 9-10 |
