| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GLEESON, Dalton | SR | Hermann High School | 4:23.80 | Verified |
| 2 | HOEKEL, Gabriel | JR | Union High School | 4:45.26 | Verified |
| 3 | BEINE, Dominick | SR | Union High School | 4:45.45 | Verified |
| 4 | KIRK, Aiden | JR | Sullivan High School | 4:54.47 | Verified |
| 5 | HALEY, Collin | JR | Pacific High School | 4:59.38 | Verified |
| 6 | TALLENT, Hunter | JR | New Haven High School | 5:05.96 | Verified |
| 7 | PERKINS, Thomas | SO | St. Clair High School | 5:12.60 | Verified |
| 8 | O'NEAL, Cameron | SR | Owensville High School | 5:12.65 | Unverified: Union Don Olszowka Invitational |
| 9 | SHIELDS, Luke | 9 | St. James High School | 5:12.96 | Verified |
| 10 | WALKER, Chase | JR | Sullivan High School | 5:15.42 | Verified |
| 11 | MONROE, Hayden | SR | Union High School | 5:17.10 | Verified |
| 12 | ZHENG, Frederic | JR | Owensville High School | 5:22.59 | Verified |
| 13 | RADEMACHER, Harm | JR | Owensville High School | 5:23.70 | Verified |
| 14 | BURNS, Joe | JR | St. James High School | 5:29.31 | Verified |
| 15 | DEARDEUFF, Zachary | SR | St. James High School | 5:38.45 | Verified |
| 16 | KINGSTON, Anthony | SR | St. Clair High School | 6:37.71 | Verified |
