| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HUNKINS, Nick | SO | Pacific High School | 5:11.79 | Verified |
| 2 | POEHLMAN, Jackson | 9 | Hermann High School | 5:13.10 | Verified |
| 3 | GUERRERO, Felix | 9 | Owensville High School | 5:16.77 | Verified |
| 4 | HOEKEL, Lucas | 9 | Union High School | 5:26.69 | Verified |
| 5 | BREWER, Jonathan | SO | St. Clair High School | 5:29.51 | Verified |
| 6 | SCHUTT, Charles | 9 | Hermann High School | 5:33.10 | Verified |
| 7 | SHOCKLEY, Trevor | SO | Owensville High School | 5:40.80 | Verified |
| 8 | BORGMANN, Aiden | 9 | Union High School | 5:43.01 | Verified |
| 9 | HARDY, Walker | SO | Sullivan High School | 5:58.55 | Verified |
| 10 | HEANEY, Aidan | 9 | Hermann High School | 6:00.32 | Verified |
| 11 | STUDDARD, Ian | 9 | Sullivan High School | 6:31.79 | Verified |
| 12 | CROCKETT, Camdon | SO | Union High School | 6:49.14 | Verified |
| 13 | WHALEY, Parker | 9 | Sullivan High School | 7:28.27 | Verified |
