| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MILLER, Morgan | JR | Hermann High School | 5:27.68 | Verified |
| 2 | MCINTYRE, Emma | SR | New Haven High School | 5:34.47 | Verified |
| 3 | GIBSON, Mekayla | SR | Owensville High School | 5:34.82 | Verified |
| 4 | BRAKEFIELD, Anna | SO | Union High School | 5:35.60 | Verified |
| 5 | LANGENBERG, Madison | SR | New Haven High School | 5:39.73 | Verified |
| 6 | UTHLAUT, Amelia | 9 | Hermann High School | 5:45.40 | Verified |
| 7 | CLARK, Jessi | SR | Union High School | 6:05.20 | Verified |
| 8 | VAUGHN, Riley | JR | Pacific High School | 6:07.61 | Verified |
| 9 | PERONA, Vanessa | 9 | St. James High School | 6:13.94 | Verified |
| 10 | HALMICK, Loren | JR | Sullivan High School | 6:15.47 | Verified |
| 11 | MEHRINGER, Olivia | 9 | Union High School | 6:18.06 | Verified |
| 12 | WEIRICH, Kyah | JR | Owensville High School | 6:29.71 | Verified |
| 13 | OTTEN, Caroline | SR | New Haven High School | 6:33.19 | Verified |
| 14 | LOEB, Hayden | JR | Owensville High School | 6:48.59 | Verified |
| 15 | SIKES, Taylor | SR | St. Clair High School | 7:05.74 | Verified |
| 16 | MACHEN, Chloe | SR | St. Clair High School | 7:35.37 | Verified |
