| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Hermann High School | A | Coffey | Hartman | Chasteen | Waechter | 1:38.30 | Verified |
| 3 | Sullivan High School | A | Goly | Farrell | Lucas | Parker | 1:36.75 | Verified |
| 4 | St. Clair High School | A | Balderson | Smith | Sanders | Reinwald | 1:36.00 | Verified |
| 5 | Owensville High School | A | Breedlove | Whelan | Whelan | Payne | 1:36.28 | Verified |
| 6 | Union High School | A | Burke | Smith | Swoboda | Kriete | 1:36.87 | Verified |
| 7 | St. James High School | A | Inda | Redburn | Gruver | Die | 1:40.15 | Verified |
