| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Union High School | A | Hoekel | Pickard | Herbst | Beine | 8:32.19 | Verified |
| 2 | Hermann High School | A | Terry | Giles | Giles | Gleeson | 8:33.95 | Verified |
| 3 | St. Clair High School | A | Kern | Perkins | Tobben | Busse | 9:07.77 | Verified |
| 4 | Owensville High School | A | O'Neal | Zheng | Rademacher | Harris | 9:08.52 | Verified |
| 5 | St. James High School | A | Garver | Deardeuff | Schultz | Shields | 9:54.80 | Verified |
| 6 | Sullivan High School | A | Thomure | Huntsman | Hardy | Whaley | 10:04.41 | Verified |
