| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Union High School | A | Brake | Tucker | Clark | Brakefield | 10:16.88 | Verified |
| 2 | Hermann High School | A | Miller | Hingst | Menke | Uthlaut | 10:28.57 | Verified |
| 3 | New Haven High School | A | Steele | Rethemeyer | Langenberg | McIntyre | 10:44.87 | Verified |
| 4 | Owensville High School | A | Branson | Gibson | Weirich | Loeb | 11:14.07 | Unverified: Union Don Olszowka Invitational |
| 5 | Pacific High School | A | Prada | Vaughn | Vaughn | Anding | 11:20.37 | Verified |
| 6 | Sullivan High School | A | Mayberry | Schatzl | Fabay | Jones | 11:31.01 | Verified |
| 7 | St. Clair High School | A | Brand | Miller | Spoon | Ella | 12:06.89 | Verified |
