Section 1 of 2
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Mehlville High School | A | Blakey | Evans | Pesselato | Wolf | DNS |
| 4 | St. Charles West High School | A | Foster | Jiles | Colyer | Williams | DNS |
| 5 | Northwest (Cedar Hill) High Scho | A | Ahmed | Robinson | Pearson | Davis | 3:59.15 |
| 6 | Francis Howell Central High Scho | A | DeVilder | Jones | Vogel | McDevitt | 4:05.56 |
Section 2 of 2
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Holt High School | A | Bryant | Looney | Dudley | Mozee | 3:42.91 |
| 2 | Timberland High School | A | McHaffie | Mclaughlin | Hill | Berndt | 3:42.09 |
| 3 | Ft. Zumwalt North High School | A | Reckel | Lee | Miller | Latina | 3:41.44 |
| 4 | Christian High School | A | Hardwicke | Ring | Hardwicke | Brackenridge | 3:36.55 |
| 5 | Francis Howell High School | A | 3:39.74 | ||||
| 6 | Farmington High School | A | Hunt | Currington | Welch | Dickinson | 3:41.52 |
| 7 | Troy Buchanan High School | A | Lollar | Love | Ballard | Benhardt | 3:42.30 |
| 8 | Francis Howell North High School | A | Alexander | Stojanovic | Miller | Thomas-Muhammad | 3:53.50 |
