| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | AKOK, Aniey | Ames High School | 1:52.95 | ||
| 2 | WALION, Quenton | Gardner Edgerton Hig | 1:54.02 | ||
| 3 | PASHEA, Baker | St. Louis University | 1:55.36 | ||
| 4 | HATTON, Aidan | Hanahan High School | 1:56.67 | ||
| 5 | TALLEY, Tanner | New Covenant Academy | 1:56.71 | ||
| 6 | DUT, Kon | Ames High School | 1:56.74 | ||
| 7 | SNOKE, Adam | Lutheran South High | 1:56.92 | ||
| 8 | WILMES, Garrett | Father Tolton | 1:58.06 | ||
| 9 | LOGAN, Romon | PACER | NT |
