| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CLARK, Makayla | Lees Summit West Hig | 2:11.59 | ||
| 2 | GOLDFARB, Kylie | John Burroughs | 2:15.60 | ||
| 3 | LESHER, Savannah | Eureka High School | 2:15.76 | ||
| 4 | MELVIN, Cheyenne | STEAM Academy at McC | 2:16.29 | ||
| 5 | MAZZEI, Deborah | Jenks High School | 2:16.93 | ||
| 6 | MAZZEI, Avery | Jenks High School | 2:17.40 | ||
| 7 | KIDDY, Zoie | Kiefer High School | 2:18.06 | ||
| 8 | SCHULTE, Ginger | Ladue Horton Watkins | 2:18.20 | ||
| 9 | RYAN, Madeleine | PACER | NT |
