| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DEATON, Jaden | Poplar Bluff High Sc | 4:17.54 | ||
| 2 | ACKLEY, Daelen | El Dorado Springs | 4:17.99 | ||
| 3 | SIMPSON, Riley | West Plains High Sch | 4:18.00 | ||
| 4 | MCDONNELL, Declan | The Tatnall School | 4:18.32 | ||
| 5 | HAUSER, Matthew | Rock Bridge | 4:18.42 | ||
| 6 | MUSICK, Grant | Kickapoo High School | 4:18.46 | ||
| 7 | MARTIN, Johnny | Ladue Horton Watkins | 4:18.94 | ||
| 8 | LITTRELL, Cale | Hickman | 4:19.08 | ||
| 9 | SCHMIDT, Sawyer | Augusta High School | 4:19.10 | ||
| 10 | SCHRAM, Ian | Festus | 4:19.28 | ||
| 11 | COTTRELL, Jack | Chattanooga Christia | 4:19.43 | ||
| 12 | KRASNOFF, Charles | Ladue Horton Watkins | 4:20.03 | ||
| 13 | HOGAN, Myles | Fordham Prep HS | 4:20.08 | ||
| 14 | CAMPBELL, Hobbs | Joplin High School | 4:20.17 | ||
| 15 | ERDKAMP, Colby | Gretna High School | 4:20.42 | ||
| 16 | SMOCK, Andrew | PACER | NT |
