| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | RUIZ, Victoria | Clear Falls High Sch | 5:07.56 | ||
| 2 | CROUCH, Cami | Nerinx Hall | 5:09.75 | ||
| 3 | THURLBY, Carolyn | Blue Valley Northwes | 5:09.97 | ||
| 4 | LOAFMAN, Mia | Rogers High | 5:10.08 | ||
| 5 | LUNA, Francie | Cor Jesu Academy | 5:13.31 | ||
| 6 | ELCAN, Abby | Har-Ber High | 5:13.55 | ||
| 7 | FISHER, Cecilia | Blue Valley Northwes | 5:13.59 | ||
| 8 | FLIEGE, Layla | Jackson | 5:15.34 | ||
| 9 | SCZUPAK, Maggie | Arlington High Schoo | 5:15.55 | ||
| 10 | JACKNEWITZ, Marisa | Nerinx Hall | 5:15.89 | ||
| 11 | SHARP, Alex | Brookfield High Scho | 5:16.64 | ||
| 12 | VOLKART, Alex | Southern Boone Count | 5:17.75 | ||
| 13 | FROST, Izzy | Rolla High School | 5:17.98 | ||
| 14 | BEVAN, Tabitha | Parkway North | 5:18.23 | ||
| 15 | WORKMAN, Alexis | The Summit Prep Acad | 5:23.44 | ||
| 16 | GIRA, Elizabeth | PACER | NT |
