| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Webster Groves High School | A | Logan | Williamson | Miller | Booth | NT | Unverified: |
| 2 | Oakville High School | A | BRADLEY | MICHEL | Francis | REDFORD | NT | Unverified: |
| 3 | DeSmet Jesuit High School | A | Kirisits | Penberthy | Peugnet | Leatherberry | 1:40.60 | Unverified: |
| 4 | Kirkwood High School | A | Johnson | Frost | Heller | Boswinkel | 1:48.00 | Unverified: SLUH Invitational |
| 5 | Mehlville High School | A | Evans | DIMMICK | Londal | Wolf | NT | Unverified: |
