| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mehlville High School | A | DUBOSE | Burton | GOMEZ | OHMER | NT | Unverified: |
| 2 | Chaminade College Preparatory Sc | A | Cavlovich | Cameron) Epps | Edwards | O'Neal | NT | Unverified: |
| 3 | DeSmet Jesuit High School | A | Gray | Thomas | Byas | Hentges | NT | Verified |
| 4 | Kirkwood High School | A | Harris | Jones | Brown | Muraski | 1:36.92 | Verified |
| 5 | Webster Groves High School | A | Fischer | Cooper | Jones | Casseus | NT | Unverified: |
