| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mehlville High School | A | NT | |||||
| 2 | DeSmet Jesuit High School | A | Leatherberry | Kirisits | Erusha | Penberthy | 4:00.00 | Unverified: |
| 3 | Ladue Horton Watkins High School | A | Cox | Loynd | Song | Tabscott | 3:30.65 | Verified |
| 4 | Kirkwood High School | A | Gjelvik | Godwin | Hassan | Kostos | 4:00.00 | Unverified: SLUH Invitational |
| 5 | Oakville High School | A | BRADLEY | MICHEL | Francis | REDFORD | NT | Unverified: |
