| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Mehlville High School | A | Londal | Silies | Blakey | DIMMICK | NT | Unverified: |
| 3 | Kirkwood High School | A | Bond | Brown | Smith | Jones | 3:40.00 | Unverified: SLUH Invitational |
| 4 | Chaminade College Preparatory Sc | A | Cattoor | Cavlovich | Hammell | O'Neal | NT | Unverified: |
| 5 | Webster Groves High School | A | Jones | Fischer | Krueger | Lack | NT | Unverified: |
