| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Webster Groves High School | A | Bronner | Jackson | Henderson | Casseus | NT | Unverified: |
| 2 | Mehlville High School | A | Burton | DUBOSE | Green | GOMEZ | NT | Unverified: |
| 3 | DeSmet Jesuit High School | A | Stewart | Gray | Key | Thomas | NT | Verified |
| 4 | Kirkwood High School | A | Harris | Welch | Smith | Jones | 46.80 | Unverified: SLUH Invitational |
| 5 | Chaminade College Preparatory Sc | A | Gray | Cameron) Epps | Marshall | Lee | DQ | Unverified: |
