| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Kirkwood High School | A | Bronstein | Patterson | Baker | Lee | 8:35.00 | Unverified: SLUH Invitational |
| 2 | DeSmet Jesuit High School | A | Pohl | Mosquera | Mosquera | Voelker | 9:11.96 | Verified |
| 3 | Chaminade College Preparatory Sc | A | Cattoor | Hammell | Hardeman | Hogenkamp | NT | Unverified: |
| 4 | Webster Groves High School | A | Bacon | Lack | Glarner | Krueger | NT | Unverified: |
| 5 | Ladue Horton Watkins High School | A | Cox | Proper | Smock | Page | NT | Unverified: |
