| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Louisiana High School | A | Jenkins | Bradley | Murray | Campbell | 4:52.18 | Verified |
| 2 | Bowling Green High School | A | Gamm | Stafford | Mcmillen | Waddell | 4:43.24 | Verified |
| 3 | Father Tolton Regional Catholic | A | Andrews | Mills | Reynolds | Sexauer | 4:23.87 | Verified |
| 4 | Mark Twain High School | A | Northcutt | Miller | Ross | Trower | 4:39.81 | Verified |
| 5 | Liberty High School | A | Davison | Kasparie | Neisen | Mitchelle | NT | Unverified: |
| 6 | Community High School | A | Angel | Johnson | Angel | Beamer | 4:53.10 | Verified |
