Section 1 of 2
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Bishop DuBourg High School | A | Harvie | Rivers | Rogers | Billiot | DQ | Verified |
| 3 | Clopton High School | A | Denslow | Martin | Hoehn | Burt | 51.92 | Verified |
| 4 | Van-Far High School | A | Eoff | Sanders | Regot | Kliewer | 1:01.25 | Verified |
Section 2 of 2
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Father Tolton Regional Catholic | A | Becvar | Hargis | Steevens | Timmerman | 49.69 | Verified |
| 3 | Louisiana High School | A | Greene | Silvey | Dzierwa | Rick) Tan | 46.97 | Verified |
| 4 | Mark Twain High School | A | Epperson | Hughes | Shepherd | Davenport | 49.46 | Verified |
| 5 | Missouri Military Academy | A | Heavens | Kazoora | Wever | Beverido | 50.06 | Verified |
