| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Van-Far High School | A | Kliewer | Hayden | Regot | Eoff | NT | Unverified: |
| 2 | Bishop DuBourg High School | A | Bradley | Harvie | Rivers | Rogers | 1:44.09 | Verified |
| 3 | Louisiana High School | A | Luck | Hutt | Greene | Dzierwa | 1:38.28 | Verified |
| 4 | Missouri Military Academy | A | Heavens | Cecil | O'Neal | Corzine | 1:44.07 | Verified |
| 5 | Father Tolton Regional Catholic | A | Willmeth | Hargis | Becvar | Yllescas | 1:44.25 | Verified |
