| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Salem MS | A | Trosper | DuBois | Kiser | Hensley | NT | Unverified: |
| 3 | Newburg Middle School | A | DQ | Verified | ||||
| 4 | Viburnum Junior High School | A | Barton | Barton | Wigger | Gibbs | 1:03.14 | Verified |
| 5 | Steelville Middle School | A | Pryor | Brown | Decker | Edgar | 1:03.45 | Unverified: Bourbon Middle School Invitation |
| 6 | Cuba Middle School | A | Hulsey | Weber | Jamieson | Viramontes | 1:10.27 | Verified |
| 7 | West County R4 | A | Smith | Province | Godat | Branham | NT | Unverified: |
