| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Newburg Middle School | A | 2:21.70 | Verified | ||||
| 4 | Steelville Middle School | A | Pryor | Decker | Mathews | Edgar | 2:07.56 | Unverified: |
| 5 | Bourbon Middle School | A | Enke | Poole | Bartle | Colon | NT | Verified |
| 6 | Cuba Middle School | A | Yates | Ringeison | Silva | Sedlak | NT | Unverified: |
