| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Newburg Middle School | A | NT | |||||
| 2 | Salem MS | A | Lindsey | Green | Diehl | Richter | NT | Unverified: |
| 3 | Viburnum Junior High School | A | Oberline | Rutledge | Rutledge | Zundel | 5:20.64 | Verified |
| 4 | Steelville Middle School | A | Cottrell | Brown | Settles | Crocker | 4:43.57 | Verified |
| 5 | Cuba Middle School | A | Smith | Mehl | Jones | Elrod | 5:01.24 | Verified |
| 6 | Stoutland MS | A | Hill | Shaw | Kincaid | Franklin | NT | Unverified: |
| 7 | Bourbon Middle School | A | Gibbs | Payne | Politte | Marlow | NT | Unverified: |
