| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Salem MS | A | Major | Mendenhall | Brakefield | Howard | NT | Unverified: |
| 4 | Cuba Middle School | A | Smith | Laney | Heads | Halbert | 55.30 | Verified |
| 5 | Steelville Middle School | A | Settles | Pounds | Martin | Craig | 1:08.28 | Unverified: Bourbon Middle School Invitation |
| 6 | Bourbon Middle School | A | Peters | Cole | Williamson | Rippeto | NT | Unverified: |
