| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Paul Lutheran School | A | Gray | Mccarty | Blackmon | Ayers | 1:03.93 | Verified |
| 2 | Cuba High School | A | Black | Herr | Moreland | Dong | 56.71 | Verified |
| 3 | St. James High School | A | Light | Satterfield | Crocker | Bullock | 55.34 | Verified |
| 4 | Rolla High School | A | Jackson | Witham | Covey | Duvel | 51.79 | Verified |
| 5 | Steelville High School | A | Perkins | Davis | Booker | Cottrell | 55.11 | Verified |
| 6 | New Haven High School | A | Sumpter | Faris | Faris | Kubiak | 56.68 | Verified |
| 7 | Vienna High School | A | Schulte | Rosentreter | Griffin | Novak | 58.20 | Unverified: Osage 9/10 Meet |
