| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Ft. Zumwalt West High School | A | 3:29.81 | Verified |
| 2 | Eureka High School | A | 3:29.13 | Verified |
| 3 | Hickman High School | A | 3:26.55 | Verified |
| 4 | Rock Bridge High School | A | 3:24.74 | Verified |
| 5 | Marquette High School | A | 3:25.80 | Verified |
| 6 | Lafayette (Wildwood) High School | A | 3:28.29 | Verified |
| 7 | Francis Howell High School | A | 3:29.26 | Verified |
| 8 | Ft. Zumwalt North High School | A | 3:33.61 | Verified |
