| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Lafayette (Wildwood) High School | A | 43.59 | Verified |
| 2 | Francis Howell High School | A | 43.46 | Verified |
| 3 | Timberland High School | A | 43.14 | Verified |
| 4 | Hickman High School | A | 42.51 | Verified |
| 5 | Christian Brothers College High | A | 43.34 | Verified |
| 6 | Ft. Zumwalt North High School | A | 43.36 | Verified |
| 7 | Troy Buchanan High School | A | 43.52 | Verified |
| 8 | Eureka High School | A | 43.84 | Verified |
