| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FORD, Carolyn | SO | Rock Bridge High School | 2:16.27 | Verified |
| 2 | REED, Mia | SR | Washington High School | 2:17.61 | Verified |
| 3 | KLEEKAMP, Leah | JR | Eureka High School | 2:18.11 | Verified |
| 4 | BAKER, Josie | 9 | Kirkwood High School | 2:18.26 | Verified |
| 5 | LESHER, Savannah | SR | Eureka High School | 2:18.86 | Verified |
| 6 | GRAY, Madelyn | SR | Ft. Zumwalt South High School | 2:22.53 | Verified |
| 7 | PARSONS, Grace | SO | Rock Bridge High School | 2:24.65 | Verified |
| 8 | LEHMBECK, Lydia | SR | Ft. Zumwalt West High School | 2:27.86 | Verified |
