| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GATES, Ellen | SR | Hickman High School | 50.29 | Verified |
| 2 | DUEKER, Kinsey | JR | Troy Buchanan High School | 49.16 | Verified |
| 3 | SHELTON, Eve | SR | Liberty (Wentzville) High School | 48.15 | Verified |
| 4 | DIXON, Genesis | SO | Kirkwood High School | 45.45 | Verified |
| 5 | ADAMS, Aliyah | JR | Timberland High School | 48.18 | Verified |
| 6 | FIGAS, Ingrid | JR | Washington High School | 48.33 | Verified |
| 7 | FIGGINS, Lily | SR | Francis Howell High School | 49.61 | Verified |
| 8 | LOPEZ, Matte | SR | Hickman High School | 50.56 | Verified |
