| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Lafayette (Wildwood) High School | A | 9:39.37 | Verified |
| 2 | Timberland High School | A | 9:48.05 | Verified |
| 3 | Rock Bridge High School | A | 9:49.89 | Verified |
| 4 | Liberty (Wentzville) High School | A | 9:50.05 | Verified |
| 5 | Eureka High School | A | 9:53.98 | Verified |
| 6 | Kirkwood High School | A | 10:02.66 | Verified |
| 7 | Ft. Zumwalt South High School | A | 10:10.15 | Verified |
| 8 | Ft. Zumwalt West High School | A | 10:28.75 | Verified |
