| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Ft. Zumwalt South High School | A | 1:49.77 | Verified |
| 2 | Hickman High School | A | 1:48.35 | Verified |
| 3 | Eureka High School | A | 1:46.83 | Verified |
| 4 | Rock Bridge High School | A | 1:44.59 | Verified |
| 5 | Francis Howell High School | A | 1:46.62 | Verified |
| 6 | Lafayette (Wildwood) High School | A | 1:47.75 | Verified |
| 7 | Kirkwood High School | A | DQ | Verified |
| 8 | Troy Buchanan High School | A | DQ | Verified |
