| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KRUGER, Ally | JR | Liberty (Wentzville) High School | 5:00.12 | Verified |
| 2 | TYSON, Grace | SO | Lafayette (Wildwood) High School | 5:04.76 | Verified |
| 3 | REED, Mia | SR | Washington High School | 5:06.44 | Verified |
| 4 | WHITAKER, Kennedy | SO | Parkway West High School | 5:10.84 | Verified |
| 5 | GRAY, Madelyn | SR | Ft. Zumwalt South High School | 5:17.83 | Verified |
| 6 | WALKER, Brooke | JR | Rock Bridge High School | 5:25.56 | Verified |
| 7 | MOORE, Madilyn | SR | Timberland High School | 5:26.07 | Verified |
| 8 | WELCH, Peri | SR | Hickman High School | 5:33.04 | Verified |
